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Figure 2.
FIG. 2. Point mutations in the JNK binding domain of JIP1
abolish JIP-JNK interaction. COS 7 cells were co-transfected
with plasmids encoding Myc-JIP1 (0.5 µg) or
JIP1(R160G/P161G) (0.5 µg) and FLAG-JNK (0.5 µg) as
indicated. Cell lysates were analyzed by immunoprecipitation
using JIP1 antibody. Immune complexes were analyzed for the
presence of JNK using anti-FLAG antibody. Cell lysates from
corresponding experiments were analyzed for equivalent
expression of JIP1 and JNK.
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