Figure 6.
Figure 6. Immunogold EM Localization of CRIPT in Rat
Brain(A) Immunogold labeling for CRIPT in CA1 region of
hippocampus (F(ab′)2, conjugated to 10 nm gold particles). The
postsynaptic portions of two asymmetric synapses are
immunopositive.(B and C) Labeling of CRIPT in layer III of
somatosensory cortex (12 nm gold in [B]; 18 nm gold in [C]).
Labeling is concentrated over the postsynaptic density but may
also be seen presynaptically.(D) Double labeling for CRIPT (12
nm gold, small dots) and PSD-95 (18 nm gold, large dots) in
layer III of somatosensory cortex showing colocalization in an
asymmetric synapse.(E) The distribution of immunogold particles
along the axo-dendritic axis exhibited maximal density vert,
similar 35 nm inside the postsynaptic membrane corresponding
roughly to the inner edge of the postsynaptic density. Smaller
peaks in density were seen at the presynaptic and postsynaptic
membranes. Dashed line marks outer leaflet of postsynaptic
membrane.(F) Lateral distribution of particle density along the
synapse. To permit comparison of locations in synapses with
differing active zone lengths, lateral positions were normalized
(see Experimental Procedures). Labeling was fairly uniformly
distributed across the synapse, declining sharply at its
edge.Scale BARS = 250 nm, (A); 100 nm, (B–D).
vert,
similar 35 nm inside the postsynaptic membrane corresponding
roughly to the inner edge of the postsynaptic density. Smaller
peaks in density were seen at the presynaptic and postsynaptic
membranes. Dashed line marks outer leaflet of postsynaptic
membrane.(F) Lateral distribution of particle density along the
synapse. To permit comparison of locations in synapses with
differing active zone lengths, lateral positions were normalized
(see Experimental Procedures). Labeling was fairly uniformly
distributed across the synapse, declining sharply at its
edge.Scale BARS = 250 nm, (A); 100 nm, (B–D).