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Figure 5.
Figure 5. Concentration of CRIPT at Excitatory Synapses in
Cultured Hippocampal Neurons(A–C) Double staining with
antibodies against PSD-95 (A) and CRIPT (B) shows that these
proteins colocalize (C) especially on dendritic spines
(arrowheads).(D) Antibodies against CRIPT stain throughout the
neuron, but immunoreactivity is concentrated in dendritic
spines.(E–H) Staining with antibodies against CRIPT (green in
[E–H]) and rhodamine phalloidin to label concentrations of
F-actin in spines (red in [F] and [H]) shows localization of
CRIPT to dendritic spines (E and F). Spiny immunoreactivity of
CRIPT is lost after preincubation of the antibody with the
immunogenic peptide (G and H).(I) Antibodies against the
presynaptic marker SV2 (red) and CRIPT (green) show closely
apposed signals, indicating that CRIPT punctate staining is
synaptic (arrowheads).(J) The lack of colocalization in the
staining for GAD (red) and CRIPT (green) indicates that CRIPT
(arrowheads) is not concentrated at inhibitory synapses
(arrows).Scale BARS = 10 μm.
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