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Figure 4.
Fig. 4. Activation of effector cells by MICA engagement or
ligation of NKG2D. (A) Expression of MICA sensitized Daudi-  [2]m
transfectants to lysis by NKL cells. Cytotoxicity was inhibited
by anti-MICA (mAb 2C10) or anti-NKG2D (mAb 1D11). Anti-HLA-E mAb
3D12 restored lysis (4). (B) The anti-NKG2D mAbs 1D11 and 5C6
induced redirected lysis of mouse mastocytoma Fc  R^+ P815
cells by NKL cells, by the  1B V[  ]1
T-cell
clone, and by a peripheral blood CD8^+  T-cell
clone (10). Data shown are representative of four and five
T-cell clones, respectively. (C) Cytotoxicity of NKL cells
against the HeLa (cervical), DU145 (prostate), HTB-78 (ovary),
and SW480 (colon) tumor cell lines was decreased by anti-NKG2D
mAb 1D11 or by F(ab')^2 fragments of the anti-MICA and anti-MICB
mAb 6D4 but not by control IgG. Inhibitions were partial, as is
often the case in antibody blocking. Data in (A) to (C)
represent reproducible averages of three to five independent
experiments and were obtained at an E:T of 10:1.
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