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Figure 3.
Fig. 3. Dependence of ACE2 proteolytic activity on anion
concentration. ACE2-catalyzed hydrolysis reactions were
performed with 0.15 nM ACE2 and 50 µM Mca-APK(Dnp) in
salt, 10 µM ZnCl[2], 0.01% Brij-35, and 50 mM MES, pH 6.5,
as described under "Experimental Procedures." Rates of
hydrolysis of the internally quenched fluorescent peptide
Mca-APK(Dnp) were determined by measuring the slope of increase
in fluorescence (excitation = 320 nm, emission = 405 nm) under
initial velocity conditions (  10%
hydrolysis) over 15-60 min. All values are an average (n = 2),
and the S.D. is shown.  , NaCl;
 , NaBr;
 , NaF;
 circle ,
KCl.
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