Figure 2 - full size

Figure 2. Figure 2. BALB/c thymocytes and stimulated splenocytes express ligands for mNKG2D. (a) Freshly isolated thymocytes from C57BL/6 and BALB/c mice were stained with monoclonal antibodies to CD4 and CD8 and with the streptavidin-phycoerythrin (PE) -complexed NKG2D tetramer (filled histogram). Staining with an irrelevant T22-tetramer (solid line) and blocking of the NKG2D-tetramer staining (dashed line) with an excess (molar ratio 5:1) of unlabeled tetramer (multimerized with streptavidin alone) was performed to show the specificity of the staining. The histograms show electronic gating on the designated cell populations. One representative experiment out of three is shown. (b) Splenocytes from C57BL/6 and BALB/c mice were stimulated for 72 h with ConA or LPS and stained with monoclonal antibodies to CD4, CD8 and CD19 and with streptavidin-PE -complexed NKG2D tetramer (filled histogram). Staining with an irrelevant T22-tetramer (solid line) and blocking of the NKG2D-tetramer staining (dashed line) with an excess (molar ratio 5:1) of unlabelled tetramer (multimerized with streptavidin alone) was performed to show the specificity of the staining. The histograms show electronic gating on the designated cell populations. One representative experiment out of four similar experiments is shown.