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Figure 2.
Fig. 2. IB1 is abundantly expressed in insulin-secreting
cells. A, five micrograms of poly(A)+ RNA prepared from two
different insulin-secreting cell lines, from rat liver, kidney,
and whole pancreas, were analyzed by Northern blotting for IB1
gene expression. B, a total of 5 µg of RNA obtained^ from
isolated rat pancreatic islets incubated in 2.8 or 30 mM glucose
for 14 h were analyzed by Northern blotting, together with rat
liver and adipose tissue RNAs. C, Western blot analysis of  TC3 whole
cell extracts with the  -IB1
antibody demonstrated^ the presence of a 120-kDa product that
was not detected with the^ preimmune serum (CTRL). D, the IB1
protein is detected in 30 µg of whole cell extracts
obtained from isolated rat pancreatic islets when analyzed by
Western blotting with the -IB1
antibody. E, a plasmid containing the IB1 cDNA driven by a CMV
promoter or its parent vector was transiently transfected into
COS-7 cells and cytoplasmic (CE) or nuclear (NE) extracts
prepared 48 h after transfection. By Western blot analysis, IB1
is detected in the^ cytoplasm and the nucleus of the transfected
cells.
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