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Figure 2.
Fig. 2. pH dependence of ACE2 proteolytic activity.
ACE2-catalyzed hydrolysis reactions were performed with 0.15 nM
ACE2 and 50 µM Mca-APK(Dnp) in 1.0 M NaCl, 10 µM
ZnCl[2], 0.01% Brij-35, and 50 mM buffer as described under
"Experimental Procedures." Rates of hydrolysis of the internally
quenched fluorescent peptide Mca-APK(Dnp) were determined by
measuring the slope of increase in fluorescence (excitation =
320 nm, emission = 405 nm) under initial velocity conditions (
 10%
hydrolysis) over 15-60 min. All values are an average (n = 2),
and the S.D. is shown.  , sodium
acetate;  , MES;
 ,
bis-Tris propane;  circle ,
CHES;  , CAPS.
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