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Figure 1.
Fig. 1. Map of pTSA-3'proc vector designed for gene
expression in procyclic forms of T. brucei (kindly provided by
D. Salmon and E. Pays). The coding sequences (PPDK: pyruvate,
phosphate dikinase; HYG: hygromycine resistance;  -TUB and
 -TUB: - and -tubulin)
are indicated by hatched boxes. The black boxes flanking the
PPDK gene contain the procyclin promoter indicated by an arrow
(5' proc) or the polyadenylation signal present in the 3'
noncoding region of the procyclin (3' proc), and the splice
leaders (SL) are shown. The white boxes represent the tubulin
intergenic region used to target the insertion of the
recombinant pTSA-3'proc (B) into the tubulin gene cluster (A),
and the pUC18 vector is shown as a hatched thin box.
Abbreviations: B, BssHII; Xb, XbaI and Xh, XhoI.
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