|
Figure 1.
Fig. 1. Sequence analysis of the evolved AspATs that have
high activity for 2-oxovaline. (A) Lines show the entire coding
region of the aspC gene. Longer bars indicate the nucleotide
positions of missense mutations, which caused amino acid
substitutions, and shorter bars indicate those of silent
mutations. Asterisks are the missense mutations conserved in all
five mutant AspATs. (B) Amino acid substitutions. Residues were
numbered according to the sequence of cytosolic AspAT from pig
as described (27).
|
