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Figure 5.
Figure 5. High-throughput phage competition ELISA for
estimating affinities of anti-mVEGF scFvs. The x axis shows the
clone number; clones 1-68 were from Lib-scFv[2] and clones 69-86
were from Lib-scFv. The y axis shows the ratio of the phage
ELISA signal (A[450]) in the presence of 100 nM mVEGF (+VEGF) to
that in the absence of solution-phase mVEGF ( -VEGF). Asterisks
indicate clones that were chosen for further analysis. See
Materials and Methods for further details.
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