Figure 5.
Figure 5 Initial damage detection by UDG Ser -Pro pinch. (A)
Backbone compression forced by three Ser -Pro-rich loops. The
loops of the Ser -Pro pinch (green) compress the
uracil-containing DNA strand at the phosphates 5' and 3' of the
uracil nucleotide in the directions indicated by the arrows. The
wtUDG/U A
structure is shown, but this compression occurs in all of the
complex structures. (B) The UDG Ser -Pro pinch for initial
damage detection. The initial UDG -DNA complex is recreated by
superimposing straight B-DNA (white) onto the kinked DNA seen in
the co-crystal structures (orange), and the structure of
uncomplexed wtUDG (stippled magenta ribbons) onto the DNA-bound
enzyme. The view is looking into the DNA major groove at the
Leu272 loop (center) and the 4-Pro loop (165 -169). These Ser
-Pro loops, along with the Gly -Ser loop (246 -247), interact
with the DNA phosphodiester backbone, compressing the
intrastrand phosphate distance of the uracil-containing DNA
strand and kinking DNA.
A
structure is shown, but this compression occurs in all of the
complex structures. (B) The UDG Ser -Pro pinch for initial
damage detection. The initial UDG -DNA complex is recreated by
superimposing straight B-DNA (white) onto the kinked DNA seen in
the co-crystal structures (orange), and the structure of
uncomplexed wtUDG (stippled magenta ribbons) onto the DNA-bound
enzyme. The view is looking into the DNA major groove at the
Leu272 loop (center) and the 4-Pro loop (165 -169). These Ser
-Pro loops, along with the Gly -Ser loop (246 -247), interact
with the DNA phosphodiester backbone, compressing the
intrastrand phosphate distance of the uracil-containing DNA
strand and kinking DNA.