Figure 4.
Fig. 4. Molecular mass analysis of Src- U by gel
filtration. The G75 gel filtration column was calibrated with
alcohol dehydrogenase^ ( circle ,
150 kDa), BSA ( , 67 kDa),
and chymotrypsinogen ( , 30 kDa) in
elution buffer (50 mM triethanolamine, pH 7.6/200 mM NaCl/5^ mM
DTT/1 mm sodium azide). Src- U ( , 0.2
mg/ml) eluted between BSA and chymotrypsinogen.
U by gel
filtration. The G75 gel filtration column was calibrated with
alcohol dehydrogenase^ ( 
circle ,
150 kDa), BSA ( 
, 67 kDa),
and chymotrypsinogen ( 
, 30 kDa) in
elution buffer (50 mM triethanolamine, pH 7.6/200 mM NaCl/5^ mM
DTT/1 mm sodium azide). Src- 
, 0.2
mg/ml) eluted between BSA and chymotrypsinogen.