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Figure 4.
Fig. 4. Cell penetration of NYAD-1 and NYAD-13 in 293T cells.
Representative confocal microscopy images of 293T cells
incubated for 20 h at 37 °C with FITC-conjugated peptides.
Upper panel: Left, differential interference contrast (DIC)
image of cells with FITC-CAI; center, FITC fluorescent image of
the same cells with FITC-CAI; and right, overlay of DIC and FITC
fluorescent images. Middle panel: Left, DIC image of cells with
FITC-β-Ala-NYAD-1; center, FITC fluorescent image of the same
cells with FITC-β-Ala-NYAD-1; and right, overlay of DIC and
FITC fluorescent images. Lower panel: Left, DIC image of cells
with FITC-β-Ala-NYAD-13; center, FITC fluorescent image of the
same cells with FITC-β-Ala-NYAD-13; and right, overlay of DIC
and FITC fluorescent images. A total of 200 cells were scored in
each treatment with FITC-CAI, FITC-β-Ala-NYAD-1 or
FITC-β-Ala-NYAD-13. The percentage of cells in the population
that exhibited the internal staining is shown at the bottom
right of each panel (P < 0.001 for FITC-CAI versus
FITC-β-Ala-NYAD-1 or FITC-β-Ala-NYAD-13).
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