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Figure 4.
Structural similarity between HinP1I and MspI (A) Schematic diagram of MspI-DNA
interactions, reproduced and modified from Xu et al. (5). Solid lines indicate direct
hydrogen bonds, dotted lines indicate water-mediated hydrogen bonds and `mc' indicates
interaction involving main chain atom. (B) Residues potentially important for DNA base
specific recognition: superimposition of residues of HinP1I (green) and MspI (cyan). (C)
Residues potentially important for catalysis: superimposition of active site residues of
HinP1I (green), MspI (cyan) and EcoRV (grey). Four residues belong to a common motif of
E......PDX[15]DXK (EcoRV), E......SDX[18]QXK (HinP1I) and E......TDX[17]NXK (MspI).
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