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Figure 4.
Fig. 4. Effect of GdnHCl on elution behavior and
molecular weights of intact decorin and protein core. Smooth
curves show refractive index detector output and superimposed
plots show molecular weight versus volume. Panel A, Superose 12
column equilibrated in TBS (solid line) or in TBS containing 2.5
M GdnHCl (broken line). From left to right, intact natural
decorin in (1) TBS and (2) GdnHCl and protein core in (3) GdnHCl
and (4) TBS. Panel B, Superose 6 column equilibrated in TBS and
samples dissolved in TBS (solid line) or in TBS containing 2.5 M
GdnHCl (broken line).
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