Figure 4 - full size

Figure 4.
FIG. 4. Structure of BF with G-AF at the postinsertion site. A, structure of BF bound to G-AF at the postinsertion site (blue) superimposed with a structure of BF bound to an unmodified base at the postinsertion site (gray). G-AF (red) adopts an anti conformation and obstructs the n+1 template base from occupying the preinsertion site that is itself disordered. B, C:G-AF base pair surrounded by electron density contoured at 3.5 and calculated using Fourier coefficients (F[obs] - F[calc]) [calc] with C:G-AF omitted from the final model. Hydrogen bonds (dashed lines) are shown accompanied with bond lengths.