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Figure 4.
Figure 4: Ulp1 is required for cell-cycle progression. a,
Cell-cycle phenotype of ulp1-ts cells. Micrographs show cell
morphology (Nomarski) after 6 h at 37 °C and, for a
different set of samples, nuclear staining (with DAPI) and
microtubule immunostaining after 8.5 h at 37 °C. Mutant
cells are enlarged compared with wild-type cells. After 8 h in
YPD medium at 37 °C, 58.5  3.5%
of mutant cells (n = 572) had large buds, 7.2 1.8%
had small buds and 34.3 4.1%
had no buds. The corresponding percentages for wild-type cells
(n = 425) were 32 1.8,
4 1.7
and 46.6 1.9.
b, FACS analysis of cells synchronized with  -factor
and released at 37 °C. 1C and 2C refers to one and two sets
of chromosomes per cell.
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