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Figure 4.
Figure 4. Conformational changes of Arg97 and the
phosphoryl groups of TDP to the stable product conformation in
the TDP-ADP bound complex. Overlay of the TMP/TDP-binding site
of the structures of TMPK in complex with TMP, ADP and AlF[3]
(red) and TDP and ADP (yellow). In the complex with bound TDP
and ADP the sidechain of Arg97 rotates (90°) around the bond
between the atoms CG and CD such that it cannot act as a clamp
to bring both nucleotides together for the backward reaction.
The figures were generated using the programs Molscript [28] and
Raster 3D [29].
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