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Figure 5.
Figure 5. RanGAP1 Is Localized at the Cytoplasmic Surface
of the NPC(A) Detection of RanGAP1 in NRK cells by
immunofluorescence microscopy. Cells were double stained with
αRanGAP1 (left) and RL1 (right) and analyzed by light
microscopy.(B) Detection of RanGAP1 by confocal
immunofluorescence microscopy of NRK cells. Cells were double
stained with αRanGAP1 (top panels) and RL1 (bottom panels).
Single sections through the equatorial plane (left) or nuclear
surface plane (right) are shown.(C) Immunogold localization of
RanGAP1 in isolated rat liver NEs. Shown are thin section
electron micrographs of isolated rat liver NEs displaying NPCs
either in cross sections (A–E) or in tangential sections
(F–H). Note that gold particles are found exclusively at the
cytoplasmic side of NPCs (arrowheads). Cytoplasmic (C) and
nuclear (N) faces of the NEs are easily distinguished by the
presence of residual chromatin (ch) on the nucleoplasmic
side.Scale bar, 100 nm.
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