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Figure 3.
Protein interface evolution of DARPin binding to the
ubiquitin-binding/oligomerization domain of NEMO. The N2C
scaffold AR as well as the three DARPins 1D5, 2A1, and 2F6 with
an affinity in the nanomolar range are shown in surface
representation. (Gray) The conserved surface of N2C; (red) the
variable protein interface. Residues in each ankyrin that
contribute to an improved affinity of the CC2-LZ domain are
highlighted in different colors: (black) hydrophobic; (red)
acidic; (blue) basic; (green) polar. All figures were prepared
using the program PyMOL (DeLano 2002).
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