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Figure 3.
Figure 3 RNase II D209N SIRAS statistics. (a) As a zero signal,
is about 0.8 (SHELXC output documentation), the SeMet
diffraction data (blue) is shown to contain anomalous signal
until its resolution cutoff, in contrast to the mutant native
data (red). (b) The Se site-occupancy profile indicates that the
asymmetric unit contains only one RNase II molecule, as RNase II
contains 16 Met residues in its sequence and residual
occupancies of 0.2 indicate noise peaks (Schneider & Sheldrick,
2002[Schneider, T. R. & Sheldrick, G. M. (2002). Acta Cryst.
D58, 1772-1779.]). (c) The space-group ambiguity was solved by
SHELXE as the density-modification `map contrast' statistics
(Sheldrick, 2002[Sheldrick, G. M. (2002). Z. Kristallogr. 217,
644-650.]) assuming space group P6[5] (red) clearly converged to
a higher value when compared with that of its hand-inverted
counterpart P6[1] (blue).
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