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Figure 3.
Fig. 3. [SeMet]tHisF is completely labelled with SeMet.
Electrospray mass spectra of tHisF (A) and [SeMet]tHisF (B) were
obtained on a TSQ7000 triple quadrupole mass spectrometer. The
labelled peaks indicate the charge states of measured m/z values
of the two proteins. [M+H]^+ indicates the mass of the two
proteins (27 723.5±3.1 Da for tHisF and 27 959±3.6 Da for
[SeMet]tHisF) as obtained after deconvolution of the measured
m/z values. The mass difference of 236 Da shows that all five
methionine residues in tHisF are completely replaced by SeMet in
[SeMet]tHisF.
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