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Figure 3.
Fig. 3. DNA binding affinity of Alba is modulated by
acetylation. Recombinant Alba binds a 50-oligomer DNA duplex in
a highly cooperative manner with an apparent dissociation
constant of 33 nM (green), whereas the native acetylated protein
(blue) also binds cooperatively, but more weakly (it has 1/30th
the affinity). Mutants of recombinant Alba in which Lys16 or
Lys17 have been changed to alanines (black) show intermediate
binding affinities, whereas changes to glutamate (red) result in
binding affinities similar to the acetylated protein. Binding
isotherms were determined in triplicate, with standard errors
indicated for each point.
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