Figure 3.
Figure 3 Stereo views of the electron density and atomic
coordinates showing wtUDG and L272A recognition of
uracil-containing and AP site DNA. The bias-reduced, [A]-weighted
2F[obs]-F[calc] electron density map, contoured at 2 (blue)
and 5 (pink),
is shown for the complexes (see text). (A) The wtUDG/U A
DNA complex. The electron density for the entire length of the
45°
bent DNA is shown, with protein and DNA atoms as yellow
(carbon), red (oxygen), blue (nitrogen) and green (phosphorus)
tubes. Pro271, Leu272 and Ser273 penetrate the DNA minor groove,
with the Leu272 side chain inserted into the DNA base stack
opposite the uracil orphan base partner, adenine. (B) The wtUDG/U
[A]-weighted
2F[obs]-F[calc] electron density map, contoured at 2 
A
DNA complex. The electron density for the entire length of the
45°
bent DNA is shown, with protein and DNA atoms as yellow
(carbon), red (oxygen), blue (nitrogen) and green (phosphorus)
tubes. Pro271, Leu272 and Ser273 penetrate the DNA minor groove,
with the Leu272 side chain inserted into the DNA base stack
opposite the uracil orphan base partner, adenine. (B) The wtUDG/U