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Figure 4.
Fig. 4. JIP-1 inhibits pre-B cell transformation by Bcr-Abl.
(A) JNK activation by v-Abl and Bcr-Abl is suppressed by JIP-1.
JNK activity was measured in an immune-complex kinase assay of^
293 cell lysates by means of a polyclonal JNK antibody and the^
substrate GST-Jun. The effect of transfection of the cells with
plasmid vectors that express v-Abl, Bcr-Abl, and the JBD of
JIP-1^ (residues 127 to 281) is presented. (B) Primary mouse
marrow cells were infected with the bi-cistronic retroviruses
illustrated^ (20). The mean density (×10^4) (±SE)
of nonadherent pre-B cells on day 10 is presented. The^ data
shown are derived from three independent experiments plated^ in
triplicate. The empty box in the first line indicates that the
vector does not contain an insert in this position. (C)
Photomicrographs of representative plates from (B) are shown.
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