|
Figure 2.
Fig. 2. (A) Diffraction quality crystals of rat liver
F[1]. (B) Scan of an SDS/PAGE gel of redissolved F[1] crystals.
Crystals of F[1] were dissolved in 50 µl of 250 mM KP[i]
plus 5 mM EDTA (pH 7.5), and 15 µg were subjected to
SDS/PAGE in cylindrical gels. (C) Capacity of redissolved
crystals to rebind to F[1]-depleted inner membrane vesicles of
mitochondria and regain sensitivity to oligomycin, an inhibitor
of oxidative phosphorylation. Where indicated, 1 µg
oligomycin was present. (D) Relative capacity of ATP and MgCl[2]
to initiate the F[1] ATPase reaction. The decrease in optical
density of NADH at 340 nm was monitored. Reaction 1 was
initiated with 4 mM ATP, and reaction 2 was initiated with 4.8
mM MgCl[2].
|
