Figure 2.
Figure 2 A. aeolicus PilT crystals yield high-quality data and
experimental phases. (a) Hexagonal PilT-ATP S
crystal, dimensions 0.1
× 0.1 × 0.02 mm. (b) Diffraction pattern from a
similar PilT-ATP S
crystal showing resolution rings at 9.4, 4.7 and 3.1 Å.
(c) Section from an A. aeolicus PilT electron-density map. To
illustrate molecular boundaries, we have chosen to present a map
calculated in P6 with solvent-flattened phases (using 50%
solvent) at 3.5 Å resolution and contoured at 1.5 .
This 15 Å slice through the PilT ring is viewed down the
crystallographic and molecular sixfold axis, with subunit
boundaries indicated by a 62 Å edge equilateral triangle.
(d) The subsequent 15 Å section. (e) A slab in the
direction perpendicular to the previous views (on the same
scale, with 62 Å ruler repeated) shows the tight lateral
packing of hexamers and their height. The necessary interactions
along the sixfold z axis that promote ordered crystal formation
are potentially mediated by a poorly ordered loop or N- or
C-terminal extension not visible in solvent-flattened
electron-density maps.
S
crystal, dimensions 
0.1
× 0.1 × 0.02 mm. (b) Diffraction pattern from a
similar PilT-ATP 
.
This 15 Å slice through the PilT ring is viewed down the
crystallographic and molecular sixfold axis, with subunit
boundaries indicated by a 62 Å edge equilateral triangle.
(d) The subsequent 15 Å section. (e) A slab in the
direction perpendicular to the previous views (on the same
scale, with 62 Å ruler repeated) shows the tight lateral
packing of hexamers and their height. The necessary interactions
along the sixfold z axis that promote ordered crystal formation
are potentially mediated by a poorly ordered loop or N- or
C-terminal extension not visible in solvent-flattened
electron-density maps.