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Figure 2.
Figure 2. Protein determinants of the Smg and Vts1 SAM domains
required for SRE binding. (a) Schematic of
fluorescein-labeled model SRE, based on the nos 3' UTR27, 28,
29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, of loop;
asterisks indicate expected Watson-Crick base pairing in the
stem. Bases required for Smg binding, bold. (b) Mutational and
SRE binding analysis of Smg(584 -763). (c) Mutational and SRE
binding analysis of full-length Vts1(1 -523). (d) Mutational and
SRE binding analysis of Vts1^SAM.
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