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Figure 2.
Fig. 2. Reversed phase HPLC testifies to a high purity
of [SeMet]tHisF. Elution of 160 μg protein was performed at 1
ml/min with an acetonitrile gradient (1.6–64%, dotted line) in
0.08% trifluroacetic acid and monitored on-line at 278 nm.
[SeMet]tHisF eluted as a symmetrical peak at 58 min and
integration of the peak areas yielded a purity of at least 98%.
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