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Figure 3.
Figure 3. The N-Terminal Domain of aPKC but Not of Par6B
Inhibits TJ Assembly(A) MDCK cells were transiently transfected
with plasmids encoding Par6B (1-125), aPKCζ (1-126), or aPKCζ
(1-126, V[62,66]A). Cells were fixed and stained for the myc
epitope and ZO-1.(B) Quantification of TJ assembly defect,
showing percentages of cell-cell contacts between transfected
cells still incomplete 6 hr after calcium readdition.(C)
Alignment of OPR motifs. Stars show aPKC residues that were
mutated. Hs, Homo sapiens; Mm, Mus musculus, Rn, Rattus
norvegicus; Sc, Saccharomyces cerevisiae.(D) aPKCζ (1-126,
V[62,66]A) does not coimmunoprecipitate with Par6B. COS cells
were cotransfected with plasmids encoding HA[3]-Par6B or
HA[3]-Par6B (102-373) and myc-aPKCζ (1-126) or aPKCζ (1-126,
V[62,66]A) as indicated. Par6B was immunoprecipitated and
blotted for the myc epitope. CL = cell lysate (1/10 of total).
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