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Figure 3.
Figure 3. Southern and Northern Blots for Detection of
HVEM–Homologous DNA and RNA in Cells and Tissues(A–C) Cell
DNAs were extracted and digested with BamHI and the fragments
separated by electrophoresis and transferred to Duralon nylon
membrane for hybridization. The DNAs were from HeLa (lanes 2),
HEp-2 (lanes 3), CHO-K1 (lanes 4), CHO-HVEM12 (lanes 5), and
Vero (lanes 6) cells. BamHI-digested plasmid, pBEC580, was also
included (lanes 7).(A) Photograph of the ethidium
bromide–stained gel.(B) Autoradiogram of the blot using the
PvuII probe indicated in (D).(C) Autoradiogram of the blot using
the EcoRI probe indicated in (D).(D) Schematic diagram of the
HVEM cDNA and fragments used to generate probes. Numbers on the
left of (A) and bands in lane 1 indicate molecular size markers
(kbp).(E) A Northern blot (Clontech) of polyadenylated RNAs
extracted from various human tissues and hybridized with a
^32P-labeled probe from the PvuII fragment indicated in (D). The
RNAs were extracted from heart (lane 1), brain (lane 2),
placenta (lane 3), lung (lane 4), liver (lane 5), skeletal
muscle (lane 6), kidney (lane 7), and pancreas (lane 8). Amounts
on the blot were normalized with respect to actin mRNA content.
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