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Figure 2.
Figure 2. In vitro interactions of the p47 PX and the p47
C-terminal SH3. a, p47 SH3 domains (the N-terminal SH3 and
the C-terminal and its mutant SH3) fused to GST were tested for
interactions with a His[6]-tagged p47 PX. b, p47 C-terminal SH3
(p47 SH3(C)) fused to GST was tested for interaction with
mutants of the His[6]-tagged p47 PX. The double-headed arrow
indicates the position of the His[6]-tagged p47 PX, which was
copurified with the GST-SH3s attached to glutathione beads. Only
the interaction between the wild type p47 PX and the wild type
p47 C-terminal SH3 was detected. Figure shown is representative
of at least three experiments.
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