|
Figure 2.
Fig. 2. Elution of HslV from a Sephacryl S-300 column. The
proteins (18 mg) obtained from DEAE-Sepharose column were loaded
on a Sephacryl S-300 column (2 × 80 cm) equilibrated with
buffer B containing 0.1 M NaCl. Fractions of 2 ml were
collected, and aliquots of them were assayed for the hydrolysis
of Cbz-Gly-Gly-Leu-AMC (A) or electrophoresed as described in
the legend to Fig. 1 (B). The closed circles indicate the
peptide hydrolysis, and the dotted line shows the protein
profile.
|
