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Figure 1.
Fig. 1. Minimal scheme for the GTPase reaction of Ras with
a hypothetical isomerization reaction preceding GTP hydrolysis.
The binding of GTP is very fast and the affinity to GTP very
high such that the protein is always saturated with nucleotide
(GDP or GTP). GTP hydrolysis and release of inorganic phosphate
have so far not been shown to be reversible reactions. Which of
the individual reaction steps is rate-limiting and thus
catalyzed by GAP is discussed in the text.
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