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Figure 2.
Figure 2. Binding of the YYF-COOH Peptide to the DegS PDZ
Domain(A) HSQC NMR resonances of the ^15N-labeled DegS PDZ
domain are shown in black. Resonances observed after addition of
a roughly equivalent amount of the YYF-COOH peptide are shown in
red. PDZ regions whose resonances shifted by more than 0.05 ppm
are colored green in the model in the inset.(B) Binding of 6 μl
aliquots of the YYF-COOH peptide (1.2 mM) to a solution of the
DegS PDZ domain (77 μM) monitored by isothermal titration
calorimetry. Both solutions contained 50 mM NaPO[4] [pH 7.5].
The black line is a fit of the experimental data to a binding
isotherm with a K[d] of 0.52 μM and N = 1.02.
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