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Figure 1.
Figure 1 Crystals of the Ffh/FtsY NG complex. Crystals grew with
a number of different morphologies. (a) Sheaves. Crystals were
grown from Ffh/FtsY NG complex at 20 mg ml-1 in 50 mM Tris pH
8.0, 200 mM NaCl, 2 mM MgCl[2], 1 mM GMPPCP equilibrated
against 1.51 M ammonium sulfate, 100 mM Tris pH 8, 100 mM
NaCl, 10 mM MgCl[2] at room temperature. (b) Needles and
sheaves. Crystals were grown from the protein used in (a)
equilibrated against 1.91 M ammonium sulfate, 100 mM bis-tris
pH 6.7, 100 mM NaCl, 20 mM MgCl[2], 4% PEG 400. Sheaves
appeared within 1-2 d; however, after 2.5-3 weeks needle
clusters appeared, coexisting with the sheaves, which then
dissolved such that after a month only needle clusters were
present in the drop. (c) A large well formed rod growing among
needle clusters. Crystals were grown from protein solution at
9.9 mg ml-1, equilibrated against 1.91 M ammonium sulfate,
100 mM bis-tris pH 6.7, 100 mM NaCl, 4% PEG 400. While the
sheaf and needle morphologies were readily reproducible, well
formed rods appeared in only a small subset of the
crystallization drops.
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