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Figure 1.
Fig. 1. A schematic representation of the expression
cassette used to produce human mast cell chymase C7S in B.
subtilis. Expression is driven by the B. subtilis aprE promoter
[28] shown as the thick black arrow. The chymase gene is
represented by the white box and downstream is the transcription
terminator from the B. amyloliquefaciens aprE gene [29] shown as
a thick black line. The DNA and protein sequence of the aprE
signal sequence (underlined) fused to the mature chymase gene is
shown below the schematic figure. Amino acids 9–30 of the
signal sequence are derived from the B. amyloliquefaciens aprE
gene.
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