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Figure 1.
Fig. 1. Structure of the NHERF PDZ1 domain bound to the
CFTR sequence QDTRL. A, sequence comparison of PDZ domains that
bind to CFTR. The indicated PDZ domains from human NHERF (5),
human NHERF2 (8), and murine PDZK1/CAP70 (12) were aligned using
MACAW (36). Absolutely conserved residues are shown as white
letters on blue background. Identical residues in four domains
are shaded in cyan. The secondary structure of NHERF PDZ1 is
indicated at the top. Conserved acidic residues proposed to
interact with Arg  1 of the
CFTR ligand are denoted by an asterisk. B, stereo view of the
NHERF PDZ1 crystal packing. Each carboxyl terminus serves as a
ligand for a neighboring PDZ1 molecule. C, ribbon diagram of the
NHERF PDZ1 domain bound to the QDTRL peptide. The strands  1- 6 are shown
in yellow, and the helices  1 and 2 are shown
in green. The peptide ligand QDTRL is shown in pink. The figure
was made using MOLSCRIPT (37) and Raster3D (38). D, surface
topology of the NHERF PDZ1 bound to the peptide QDTRL. The
figure was generated using GRASP (39).
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