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Figure 8.
FIG. 8. Proposed mechanism for chain length determination
catalyzed by OPPs. The first DDXXD motif attached to helix D
represents the FPP binding site. Ser-77 and Ala-76 are located
in immediate proximity of FPP, and V73Y is further down. The
substitution of Ser-77 and Ala-76 with larger residues led to
the formation of C[20], a single condensation between the bound
FPP and IPP. V73Y mutation results in temporary accumulation of
C[30]. Phe-132 located on the bottom of helix D blocks further
chain elongation of OPP and determines the ultimate product
chain length.
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