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Figure 7.
Figure 7. Comparison of Charge Distribution in Calcium
Bound G Domains(A) The G5 domain of the a2 chain of laminin
(Hohenester et al., 1999). Basic residues important in binding
of a-DG to laminin are labeled green.(B) Calcium-bound NMR
structure of the agrin-G3 B0 domain.(C) Calcium-bound B8 X-ray
structure.(D) Calcium-bound B11 X-ray structure. The calcium
binding site is indicated in the laminin structure, but is left
out for clarity in the agrin structures (conserved red cavity).
The GRASP diagrams were made using the "full.crg" charge/radius
file and are colored according to an electrostatic potential
ramp ranging from +5 e kT -1 (blue, positive) to -5 e kT -1
(red, negative). The calcium ion was not included in
electrostatic calculations. The position of the calcium binding
site (conserved red cavity) is indicated in the laminin
structure.
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