|
Figure 6.
The triple mutant RGS2(C106S,N184D,E191K), but not wild type
RGS2, inhibits dopamine D2-receptor influence on
forskolin-stimulated cAMP production. HEK293T cells were
transiently co-transfected with expression vectors for the
GloSensor cAMP biosensor and the G[i]-coupled dopamine
D2-receptor with empty vector, wild type RGS2, or the
RGS2(triple) mutant. Inhibition of forskolin-stimulated cAMP
production was determined after activation of the D2 receptor
with various concentrations of quinpirole as indicated. The
IC[50] (95% CI) for quinpirole was determined to be 18
(12–26), 14 (9–22), and 762 (498–1170) nm in the presence
of empty vector, wild type RGS2, and the triple mutant,
respectively. Inset, post-transfection cell lysates were
immunoblotted with anti-HA epitope tag antibody to confirm the
equivalent overexpression of HA-RGS2 and
HA-RGS2(C106S,N184D,E191K) proteins.
|
