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Figure 6.
Fig. 6. Stereo views depicting the sequences of the
reaction pathway catalyzed by PTP1B. A, PTP1B apoenzyme, with
WPD loop open. B, PTP1B C215S Tyr(P)-Michaelis complex, with WPD
loop closed; Gln-262 swings out of the catalytic site. C, PTP1B
Q262A cysteinyl-phosphate intermediate complex, with WPD loop
closed. D, PTP1B vanadate complex, representing the transition
state of cysteinyl-phosphate hydrolysis, with WPD loop closed;
Gln-262 swings back into the catalytic site. E, PTP1B
tungstate-product complex, with WPD loop open.
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