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Figure 5.
Interaction sites for potential binding partners
highlighted on a fully glycosylated UL18 model. (A)
Space-filling representation of the UL18/LIR-1 complex (UL18 in
magenta, β2m in slate, peptide in light green, LIR-1 in cyan)
with a complex carbohydrate model (yellow) attached to each of
the 13 potential N-glycosylation sites. The single predicted
O-glycosylation site (residue 281) within the UL18 ectodomain is
indicated by an orange sphere. Two to three additional
O-glycosylation sites are predicted in the region C-terminal to
the UL18 ectodomain fragment that was crystallized, but these
sites would be distant from the binding sites for all potential
UL18 binding partners. The UL18 counterparts of the approximate
binding sites on a class I MHC molecule for a TCR or KIR are
indicated by arrows. (B) Top view of the fully glycosylated UL18
peptide binding platform. (C) Potential US2 binding site (dark
green) on fully glycosylated UL18. (D) Potential CD8 binding
site (dark green) on fully glycosylated UL18. An enlarged view
of the CD8-binding loop in the class I MHC α3 domain (gray) is
shown with the counterpart UL18 loop (magenta).
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