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Figure 5.
Fig. 5. The effect of A-site substrate binding on the
conformation of large ribosomal subunits containing the mutation
G2581A. (a) Comparison of the conformation of the 2581 region of
wild-type ribosomes (gray) with that of the G2581A mutant
(green). Also included in the figure is CC-puromycin (blue
green) as reference for the binding site of amino acylated tRNA
to the A-site. (b) Comparison of the structure of G2581A mutant
(green) and CC-puromycin bound to a large ribosomal subunit of
wild type (gold). (c) Comparison of the structures of G2581A
mutant (green) and of CC-puromycin bound to G2581A (khaki) with
overlaid (F[G2581A]−F[G2581A CC-puromycin]) difference
electron density, which was computed by using as amplitudes the
differences observed between the data obtained from G2581A
crystals that include the analog and the data obtained from
G2581A crystals that lack the analog. Positive features were
contoured at + 4σ (blue), and negative features were contoured
at − 4σ (red).
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