Figure 5.
Figure 5 UTP-specificity and UMP binding. (A) Interactions of
the UTP sugar moiety at site A with the 2'-OH and the side
chains of N277 and S278. (B) The uridine base is specifically
recognized by Wat1 and D421 and E424. The two hydrogen atoms of
Wat1 shown as white spheres makes exquisite hydrogen bonds with
O 1
of D421 and O epsilon
1 of E424. The latter two residues are conserved among all
trypanosomatid TUTases (Figure 1). (C) UMP binding at site B;
(D) UMP binding at site C, which is located far from the active
site.
1
of D421 and O 
epsilon
1 of E424. The latter two residues are conserved among all
trypanosomatid TUTases (Figure 1). (C) UMP binding at site B;
(D) UMP binding at site C, which is located far from the active
site.