Figure 5.
Figure 5 Hin−DNA interactions in models of T11A and A10T. (A)
Energy-minimized model of T11A. The T21 and T22 methyl groups
are rendered as van der Waals spheres. The T21-methyl occludes
W2 but may stabilize W1. (B) Substitution of a T:A base pair at
position 10 in mutant A10T results in a clash with the Ser174
side chain. The van der Waals surfaces of T10−C5A and Ser174-O
are
rendered. Substitution of a C:G base pair in mutant A10C also
results in a clash with the Ser174 side chain (not shown). Note
that saturable binding was never achieved with A10T or A10C,
even at native Hin or Hin-DBD concentrations exceeding 1 M.
Models were generated by visually fitting the substituted base
pair into the Br18 structure, and for T11A, energy minimized
using CNS (Brünger et al., 1998).
are
rendered. Substitution of a C:G base pair in mutant A10C also
results in a clash with the Ser174 side chain (not shown). Note
that saturable binding was never achieved with A10T or A10C,
even at native Hin or Hin-DBD concentrations exceeding 1 
M.
Models were generated by visually fitting the substituted base
pair into the Br18 structure, and for T11A, energy minimized
using CNS (Brünger et al., 1998).