Figure 5 - full size

Figure 5.
Fig. 5. Reversed-phase HPLC chromatograms for the heme isolated from HPII catalase (A) and the mutant variants H392Q (B), H392E (C), Q419H (D), and S414A (E). The peaks of heme d are indicated by d and the peak of heme b is indicated by b. The faster eluting peak of heme d is the cis isomer and the slower eluting peak is the trans isomer.