Figure 4.
FIGURE 4. Stereo diagrams showing INH bound in the active
site mutants of sAPX W41A and H42A. A, in sAPX·(W41A),
two molecules of INH (brown) are bound in the distal cavity, one
in the same position as sAPX and a second coordinated directly
to the heme iron. The figure shows observed F[o] - F[c]
difference density (in green, contoured at 3 ). B, in sAPX (H42A)
the orientation of INH (brown) is rotated relative to the wild
type and is held in position by a hydrogen bond to Trp-41.
Observed F[o] - F[c] difference density is shown in green. In
both cases the occupancy of the INH is partial and shared with
water molecules that are represented as red spheres.
). B, in sAPX (H42A)
the orientation of INH (brown) is rotated relative to the wild
type and is held in position by a hydrogen bond to Trp-41.
Observed F[o] - F[c] difference density is shown in green. In
both cases the occupancy of the INH is partial and shared with
water molecules that are represented as red spheres.