Figure 4.
Figure 4 Exo84 and Sec5 have overlapping binding sites on the
active RalA. (A) Superposition of the Exo84-RBD:RalA and the
Sec5-RBD:RalA complexes. RalA is green in the Exo84-RBD:RalA
complex and purple when in complex with Sec5-RBD. Exo84-RBD and
Sec5-RBD are colored in red and blue, respectively. The two RalA
molecules are superimposed using all equivalent C atoms
except for residues in the two switch regions. Note that Exo84
and Sec5 cannot bind to RalA simultaneously. Close-up views of
the areas that are indicated by red and blue boxes are shown in
panels B and C, respectively. (B) Close-up view of the complex
interface around RalA switch II where significantly different
RalA conformations were observed between the two complexes.
Shown are the five RalA residues in this region that directly
contact Exo84-RBD. The molecules are colored as in panel A. (C)
Close-up view of the Sec5-RBD:RalA interface. Shown are the five
RalA residues that form hydrogen bonds with Sec5-RBD. The color
scheme is the same as in panels A and B. (D) Molecular surface
of RalA when it is in complex with Exo84-RBD. The RalA residues
that exclusively contact Exo84-RBD are colored red, the residues
that only bind Sec5-RBD are colored blue and the residues that
are involved in interactions with both effectors are colored in
orange.
atoms
except for residues in the two switch regions. Note that Exo84
and Sec5 cannot bind to RalA simultaneously. Close-up views of
the areas that are indicated by red and blue boxes are shown in
panels B and C, respectively. (B) Close-up view of the complex
interface around RalA switch II where significantly different
RalA conformations were observed between the two complexes.
Shown are the five RalA residues in this region that directly
contact Exo84-RBD. The molecules are colored as in panel A. (C)
Close-up view of the Sec5-RBD:RalA interface. Shown are the five
RalA residues that form hydrogen bonds with Sec5-RBD. The color
scheme is the same as in panels A and B. (D) Molecular surface
of RalA when it is in complex with Exo84-RBD. The RalA residues
that exclusively contact Exo84-RBD are colored red, the residues
that only bind Sec5-RBD are colored blue and the residues that
are involved in interactions with both effectors are colored in
orange.